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rabbit anti human zo 1 antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti human zo 1 antibody
    Rabbit Anti Human Zo 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+zo+1+antibody/ZO-1+Rabbit+mAb/pm41053843-124-87-95
    Average 97 stars, based on 415 article reviews
    rabbit anti human zo 1 antibody - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Efficacy of non-psychotropic Cannabis sativa L. standardized extracts in a model of intestinal inflammation.
    Article Snippet: .. After a first wash with 500 μL of PBS 1X, the cells were allowed to fix for 15 min with 500 μL of PBS 1X/ PFA 4%; then, after three wash of 5 min each (PBS 1X), the membranes were cut from the Transwell® support and moved into a new well, where 500 μL of blocking buffer (1X PBS with 5% BSA, 0.3% Triton X-100) were added for 1 h. At the end of the incubation period, the buffer was discarded, and 400 μL of the primary rabbit anti-human ZO-1 antibody (1 mg/mL, cat. #13663, Cell signaling, Danvers, MA, USA), with dilution of 1:400, was added and incubated overnight at 4 °C. .. The following day, after three new rinses of 5 min each with PBS 1X, the membranes were incubated with 400 μL of the fluorochrome AlexaFluor® 647-conjugated anti-rabbit antibody (1 mg/ mL, cat. #8889S, Cell signaling, Danvers, MA, USA), with a dilution of 1:800, for 2 h. Eventually, after other three rinse with PBS 1X, they were mounted on the glass slides using the ProLong Gold Antifade DAPI (Cell Signaling, Danvers, MA, USA), used as a fixative and nuclei stainer.

    Article Title: Efficacy of non-psychotropic Cannabis sativa L. standardized extracts in a model of intestinal inflammation
    Article Snippet: .. After a first wash with 500 μL of PBS 1X, the cells were allowed to fix for 15 min with 500 μL of PBS 1X/PFA 4%; then, after three wash of 5 min each (PBS 1X), the membranes were cut from the Transwell ® support and moved into a new well, where 500 μL of blocking buffer (1X PBS with 5% BSA, 0.3% Triton X-100) were added for 1 h. At the end of the incubation period, the buffer was discarded, and 400 μL of the primary rabbit anti-human ZO-1 antibody (1 mg/mL, cat. #13663, Cell signaling, Danvers, MA, USA), with dilution of 1:400, was added and incubated overnight at 4 °C. .. The following day, after three new rinses of 5 min each with PBS 1X, the membranes were incubated with 400 μL of the fluorochrome AlexaFluor ® 647-conjugated anti-rabbit antibody (1 mg/mL, cat. #8889S, Cell signaling, Danvers, MA, USA), with a dilution of 1:800, for 2 h. Eventually, after other three rinse with PBS 1X, they were mounted on the glass slides using the ProLong Gold Antifade DAPI (Cell Signaling, Danvers, MA, USA), used as a fixative and nuclei stainer.

    Incubation:

    Article Title: Efficacy of non-psychotropic Cannabis sativa L. standardized extracts in a model of intestinal inflammation.
    Article Snippet: .. After a first wash with 500 μL of PBS 1X, the cells were allowed to fix for 15 min with 500 μL of PBS 1X/ PFA 4%; then, after three wash of 5 min each (PBS 1X), the membranes were cut from the Transwell® support and moved into a new well, where 500 μL of blocking buffer (1X PBS with 5% BSA, 0.3% Triton X-100) were added for 1 h. At the end of the incubation period, the buffer was discarded, and 400 μL of the primary rabbit anti-human ZO-1 antibody (1 mg/mL, cat. #13663, Cell signaling, Danvers, MA, USA), with dilution of 1:400, was added and incubated overnight at 4 °C. .. The following day, after three new rinses of 5 min each with PBS 1X, the membranes were incubated with 400 μL of the fluorochrome AlexaFluor® 647-conjugated anti-rabbit antibody (1 mg/ mL, cat. #8889S, Cell signaling, Danvers, MA, USA), with a dilution of 1:800, for 2 h. Eventually, after other three rinse with PBS 1X, they were mounted on the glass slides using the ProLong Gold Antifade DAPI (Cell Signaling, Danvers, MA, USA), used as a fixative and nuclei stainer.

    Article Title: Efficacy of non-psychotropic Cannabis sativa L. standardized extracts in a model of intestinal inflammation
    Article Snippet: .. After a first wash with 500 μL of PBS 1X, the cells were allowed to fix for 15 min with 500 μL of PBS 1X/PFA 4%; then, after three wash of 5 min each (PBS 1X), the membranes were cut from the Transwell ® support and moved into a new well, where 500 μL of blocking buffer (1X PBS with 5% BSA, 0.3% Triton X-100) were added for 1 h. At the end of the incubation period, the buffer was discarded, and 400 μL of the primary rabbit anti-human ZO-1 antibody (1 mg/mL, cat. #13663, Cell signaling, Danvers, MA, USA), with dilution of 1:400, was added and incubated overnight at 4 °C. .. The following day, after three new rinses of 5 min each with PBS 1X, the membranes were incubated with 400 μL of the fluorochrome AlexaFluor ® 647-conjugated anti-rabbit antibody (1 mg/mL, cat. #8889S, Cell signaling, Danvers, MA, USA), with a dilution of 1:800, for 2 h. Eventually, after other three rinse with PBS 1X, they were mounted on the glass slides using the ProLong Gold Antifade DAPI (Cell Signaling, Danvers, MA, USA), used as a fixative and nuclei stainer.



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